The Tutorial of MHC2MIL Predictions

For the time being, this server online can predict peptides binding to 26 MHC class II molecules. The steps on how to make a prediction and what is the meaning of the result are as below.

A. How to obtain predictions

This is a screenshot of the input page. The numbers in red are related to the referring sections below.

the components of the main frame the main frame press the button to process the prediction
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1. Input the protein sequence(s)

In the first place, you should specify the sequence(s) which you want to scan for the binding peptides. The sequences can either be typed directly into the text area, or can be uploaded from a file using the button. If both the text area and the file uploaded containing sequences, this server will treat them both as inputs and the sequence(s) in the uploaded file will be appended to the data in the text area.

Two types of formats are acceptable to this server:

By FASTA Format, long antigenic protein sequences in one or multiple lines, each of which begins with '>', are allowed. The lines starting with '>' are treated as the names of the following sequences. In prediction, these sequence are split into peptides with the fixed length you specify. The other format, i.e. PEPTIDE, you can input peptides with variable length (at least 9) directly. Then the length option has no sense for the server will give direct predictions for these variable-length peptides.

All sequences have to be amino acids specified in single letter code (ACDEFGHIKLMNPQRSTVWY) and other letter code (BUXZ*) will be pointed out before being ignored by the input processor. The server will ignore any other non-white space characters beside them. If the number of the legal characters in a sequence is under nine, this sequence will be ignored for too short as a binding peptide.

If you have trouble in choosing protein sequences or deciding the suitable format, the web page provides examples for each format. Please click "show an example" to provide an example for the selected format.

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2. Input MHC molecules

The target species can be chose in the first list box. For now only homo species is available.

In the second list box, you can input more than one alleles at a time, the alleles should be space or comma separated. For example,

"HLA-DRB1_0101 HLA-DRB1_0301 HLA-DRB1_0401" or "HLA-DRB1_0101, HLA-DRB1_0301, HLA-DRB1_0401"

    Simply, you can enable the checkbox above and click "show an example" in the server page. A example sequence is ready for you. Feel free to try and summit.

In the third box, you can choose the length of the peptides in the protein.

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3. Choose output interface

This server provides two types of output interfaces for different users. The "Webpage" interface which is friendly to ordinary users will show all results on a dynamic web page implemented with javascript and JSP technology. The "Text" interface provides results directly in a plain text format which is friendly to computer programs.

When all the above have been done, press the "Submit" button to process the prediction.

The time cost of the prediction depends on the number of input data points. The time having passed during the prediction will be shown on the web page.

the state of processing

B. Interpreting prediction output

When the prediction has finished, the processing page will automatically change to the result page.

the discription of the result page the result page
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1. Information about prediction

The board shows some basic information during the prediction such as which alleles, the size of the input and how long the prediction processed.

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2. The sequences in the input

Each row contains the name and the full sequence of the referring protein.

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3. Scores of predictors

The scores are IC50. Lower scores means more likely stronger binding between the peptide and allele molecule.

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4. Sorting of prediction results

If your browser supports javascript, the Dynamic Table will work. Then you can click the column headers to sort the table and right click the column headers to choose the columns shown on the page.

Sort the table by the scores of DRB1*01:01 sort the table
Choose the columns choose the columns of the table